Modern Biomolecular Crystallography II
W.M. Keck Foundation Center for Molecular Structure
a CSUPERB core facility
California State University Fullerton
June 22-27, 2008
Sponsored by
Bruker-AXS, Emerald BioSystems, GE Healthcare, Hampton Research, iLinc Communications, Korima, MITeGen,
Molecular Dimensions, Molsoft, Oxford Diffraction Systems/Varian, Rayonix/MarUSA, Rigaku Americas Corp
Tentative Schedule of Events ( last updated 06/17/2008 05:12:12 PM -0700)
Sunday, June 22, 2008
Meet in McCarthy Hall 501
10:00am Coffee
10:30am Welcome
Katherine Kantardjieff, CMolS
Steven Murray, Dean
College of Natural Science and Mathematics, CSUF
10:45 – 11:00am Workshop Overview
Katherine Kantardjieff, CMolS
11:00 – 12:30n Principles of X-ray Diffraction I - Fundamentals
Bernhard Rupp, qed life science discoveries; Clyde Smith, SSRL
12:30 – 1:45pm Lunch
your choice; within walking or driving distance of campus; maps provided
1:45 – 2:45pm Principles of Protein Production for Crystallography
Katherine Kantardjieff, CSUF
2:45 – 3:00pm Break
3:00 – 4:00pm Principles of Protein Crystallization
Bernhard Rupp, qed life science discoveries
4:00 – 5:15pm Protein Crystallization Practical (moves to DBH - 161)
Bernhard Rupp, qed life science discoveries; Katherine Kantardjieff CMolS
5:15 – 5:30pm Wrap-up and Q&A
5:30pm Adjourn; Dinner at Chomp Sushi - 7pm
Monday, June 23, 2008
Meet in McCarthy Hall 501
8:00am Continental Breakfast
8:30 – 9:30am Instrumentation and Software for Biomolecular Crystallography
Eric Hnath, Rigaku MSC
9:30 – 10:15am Principles of X-ray Diffraction II - Phase Determination of Macromolecules
Bernhard Rupp, qed life science discoveries; Clyde Smith, SSRL
10:15 - 10:30am Break
10:30 – 11:15am Principles of X-ray Diffraction II - Phase Determination of Macromolecules
Bernhard Rupp, qed life science discoveries; Clyde Smith, SSRL
11:15 – 12:15 Electron Density Maps, Density Modification and Coot
Bernhard Rupp qed life science discoveries, Katherine Kantardjieff CMolS
12:15n – 1:30pm Lunch
1:30 – 2:30pm More Electron Density Maps, Density Modification and Coot
Bernhard Rupp qed life science discoveries, Katherine Kantardjieff CMolS
2:30 – 3:30 pm Data Collection I: Strategies
Clyde Smith, SSRL
3:30 – 3:45pm Break
3:45 – 5:15pm Data Collection II: Remote Access to Synchrotron Beamlines
Clyde Smith, SSRL
5:15 – 5:30pm Wrap-up and Q&A
5:30pm Adjourn; Dinner at BJ's Brewhouse - 7pm
Tuesday, June 24, 2005
Meet in McCarthy Hall 501
8:00am Continental Breakfast
8:30 – 9:45am Data Collection III: Assessment, Scaling, Reduction
Clyde Smith, SSRL
9:45 – 10:45am Remote Data Collection on In-House Instrumentation
Chris Ceccarelli, Oxford Diffraction Systems
10:45 - 11:00am Break
11:00am – 12:30pm Refinement of Macromolecules
Bernhard Rupp qed life science discoveries, Katherine Kantardjieff CMolS
12:30 – 1:45pm Lunch
1:45 – 3:00pm Crystal Inspection; Crystal Mounting; Cryoprotection (relocate to DBH-175)
Katherine Kantardjieff CSUF; Clyde Smith SSRL
3:00 – 3:15pm Break
3:15 – 5:15pm Protein Production Laboratory Session 1 (DBH-161)
Blake Gillespie, CSUCI
5:15 – 5:30pm Wrap-up and Q&A
5:30pm Adjourn; Dinner at Amazon Churrascaria - 7pm
Wednesday, June 25, 2008
Meet in McCarthy Hall 501
8:00am Continental Breakfast
8:30 – 9:30am Validation and Analysis of Macromolecules
Bernhard Rupp qed life science discoveries, Katherine Kantardjieff, CMolS
9:30 – 12:00n Protein Production Laboratory Session 2 (DBH-161)
Blake Gillespie, CSUCI
12:00 – 1:15pm Lunch
1:15 – 2:45pm Protein Production Laboratory Session 3 (DBH-161)
Blake Gillespie, CSUCI
2:45 – 3:00pm Break
3:00 – 4:00pm Protein Characterization: NMR, CD, DLS, AUC (relocate back to MH-501)
Katherine Kantardjieff, CMolS
4:00 – 5:15 pm Publishing Macromolecular Structures I: The Protein Data Bank and Structure Deposition
Katherine Kantardjieff, CMolS
5:15 – 5:30pm Wrap-up and Q&A
5:30pm Adjourn; Dinner at Don Jose Mexican Restaurant - 7pm
Thursday, June 26, 2008
Meet in McCarthy Hall 501
8:00am Continental Breakfast
8:30 – 10:30am Using Crystallographic Information I: Structural Bioinformatics, Modeling, Visualization and Computational
Biochemistry
Katherine Kantardjieff, CMolS
10:30 - 10:45am Break
10:45 - 12:15n Using Crystallographic Information II: Structure - Based Drug Discovery/Design
James Zhao, CMolS
12:15 – 1:30pm Lunch
1:30 – 3:00pm Other Methods: Neutron diffraction, fiber diffraction
Katherine Kantardjieff, CSUF
3:00 – 3:15pm Break
3:15 – 5:15pm Open Laboratory (DBH-161,175; MH-501)
Katherine Kantardjieff, CMolS; Bernhard Rupp, qed life science discoveries; Clyde Smith, SSRL;
Qiang (James) Zhao, CMolS
5:15 – 5:30pm Wrap-up and Q&A
5:30pm Adjourn; Dinner at Heroes - 7pm
Friday, June 27, 2008
Meet in McCarthy Hall 501
8:00am Continental Breakfast
8:30 – 9:30am Research Resources for Crystallography
Katherine Kantardjieff, CMolS
9:30 – 10:30am Teaching and training resources, strategies and approaches
Katherine Kantardjieff, CMolS; Bernhard Rupp, qed life science discoveries
10:30 – 10:45am Break
10:45 – 12:15pm Open Discussion
12:15 – 12:30pm Wrap-up and Q&A
12:30pm Adjourn